Blog entry by Samir NEGGAZ

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SamirNeggaz andZohraFortas

Laboratory of Biology ofMicroorganismsand Biotechnology(LBMB),Departmentof Biotechnology,Faculty ofSciences, Universityof
Es-Sénia, BP1524 El M’naouer,Oran 31000,Algeria

 

 

Received:
October
23, 2012/Accepted:December 15, 2012/ Published: March 30,2013.

 

 

Abstract:Inthepresentpaper,theethylacetateextractfromthefruitingbodiesofTirmaniapinoyi(Maire)wasobtainedbySoxhletextraction.Sixfractionswereseparatedfromthisextractusingtwochromatographicmethods.AllthesefractionsweresubmittedtoantimicrobialactivityagainstfourclinicallyimportantbacteriaStaphylococcusaureusATCC6538,EnterococcusfaecalisATCC6538,PseudomonasaeruginosaATCC14028,EscherichiacoliATCC25922andonepathogenicfungusCandidaalbicansATCC10231.Thein vitroantimicrobial activitywasperformedbyagar discdiffusionmethod.Thefractionswith the greatestantimicrobial activitywerefractions02and06whichinhibitedgrowthofbothGramnegativeandGrampositivebacteriaandhadsignificantantifungalactivityagainstCandidaalbicans.Thepresentstudyvalidatesthefolkuseoftheboiledtrufflewater-extractandindicatesthatitcould be
effective
potential candidatesfor thedevelopment ofnew strategiesto treatbacterialor fungal infections.

 

Key words:Agardiffusionassay,Tirmaniapinoyi,antimicrobialactivity,chromatographic methods, Soxhletextraction.