mohammed benyamina
Articles de blog de mohammed benyamina
Tout le monde (grand public)
The distribution of a-melanocyte-stimulating hormone (a-MSH) in the central nervous system of the frogRana ridibunda was determined by immunofluorescence using a highly specific antiserum, a-MSH-like containing perikarya were localized in the infundibular region, mainly in the ventral hypothalamic nucleus. A rich plexus of immunoreaetive fibers directed towards the ventral telencephalic region was detected.
Reverse-phase high-performance liquid chromatography and radioimmunoassay were used to characterizea-MSH-like peptides in the frog brain. Chromatographic separation revealed that immunoreaetive a-MSH coeluted with synthetic des-Na-acetyl a-MSH, authentic a-MSH and their sulfoxide derivatives. The heterogeneity of a-MSH-like material in the frog brain was in marked contrast with the figure observed in the intermediate lobe of the pituitary gland where only des-Na-acetyl a-MSH ispresent. These findings support the existence of discrete a-MSH immunoreactive neurons in the frog brain containing both desacetyl and authentic a-MSH.