Articles de blog de Adel Amar AMOURI
Abstract
Four expressed sequence tag-simple sequence repeat (EST-SSRs) primers were used to show genetic variability in nine accessions of M. truncatula comparing with two contrasting Medicago truncatula genotypes (Tru 131, tolerant and Jemalong, sensitive). The amplification of the DNA isolated from individual seedlings for each genotype with four primers (MTIC 044, MTIC 124, MTIC 077 and MTIC 335) produced a total of 24 alleles. The sizes of the alleles detected ranged from 100 to 300 bp. These EST-SSRs markers were more polymorphic than the MTIC 044 (low polymorphism). The average of 6 alleles per primers was detected and gave moderate values of polymorphic information content (PIC) ranged from 0.12 to 0.49 and genetic diversity (Hi) from 0,13 to 0,48. The clustering analysis showed that the Algerian ecotypes (Tru 1, Tru 2, Tru 3 and Tru 4) are associated with (Tru 5 and Tru 9) Syrian and Jordanian ecotypes respectively and Tunisian ecotypes, some of these genotypes are associated with the tolerant ecotype (Tru 131 DZA).